Anterior cruciate ligament (ACL) injuries are becoming increasingly common. They often result in functional instability, which negatively affects the patient’s quality of life. After diagnosis, ACL injuries are typically treated with ACL reconstruction surgery in active patients. There are 3 commonly used grafts for ACL reconstruction surgery: patellar tendon autografts, hamstring autografts, and Achilles allografts. This article explores the critical role of the PA during ACL reconstruction surgery, particularly in efficient and precise graft preparation. By acquiring the necessary skills, PAs can be invaluable to the surgical team and to achieving positive patient outcomes.